Fig. 4. Localizations of the IQGAP family in PC12 cells. (A) Total extracts from the indicated siRNA-transfected PC12 cells were collected after the treatment with NGF for 48 hours, and were subjected to immunoblot analysis with the indicated antibodies. The expression levels of IQGAP1 and IQGAP3 were decreased specifically. The results shown are representative of five independent experiments. (B) PC12 cells were treated with NGF for 48 hours, followed by immunostaining with the indicated anti-IQGAP antibodies and Texas Red-phalloidin. The regions in white boxes are shown magnified in the far right panels. The results shown are representative of five independent experiments. Bar, 20 µm.