Fig. 2. Immunisation against the NTD of the NMDA receptor NR1 subunit prevents the neurotoxic effect of endogenous and exogenous tPA. (A) Excitotoxic lesions were performed, 4-5 days after the last inoculation, by injecting NMDA (10 nmol) into the striatum. After 30 minutes, tPA (1 mg/kg) or vehicle was injected over 15 minutes. Brains were harvested 24 hours later, and cerebral lesions were measured by analysing thionine-stained cryostat sections (see representative section for each group). Lesion volume was analysed by a two-way ANOVA and Bonferroni/Dunn (n=9-11 mice per group, mean ± s.e.m.; ^**P<0.01). (B,C) Focal permanent ischemia was induced by MCA occlusion and cerebral lesions were measured after 24 hours of injury. (B) Representative staining and (C) lesion volumes analysed by Student's t-test (n=7-8 mice per group, mean ± s.e.m.; ^*P<0.05).