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Figure 1


Fig. 1. Wnt-5a and Wnt-3a activate Dvl in dopaminergic cells. (A) Time course of Wnt-5a- and Wnt-3a-induced activation of Dvl2 and Dvl3. SN4741 cells, treated with Wnt-3a (100 ng/ml) or Wnt-5a (100 ng/ml), were lysed after 0, 1, 5, 10, 15, 30, 60 and 120 minutes. (B) Both Wnt-5a and Wnt-3a activated Dvl2 and Dvl3, but only Wnt-3a activated beta-catenin after 2 hours of stimulation. (C) The effects of Wnt-5a or Wnt-3a (100 ng/ml) on Dvl were blocked by Fz8-CRD-conditioned medium. (A-C) Phosphorylation of Dvl isoforms were detected as phosphorylation-dependent mobility shifts of total Dvl2 and Dvl3. The position of dephosphorylated ({blacktriangleleft}) and phosphorylated Dvl ({triangleleft}) is indicated. The activation of beta-catenin was determined by western blotting using antibodies against active (Ser33-Thr41-dephosphorylated) beta-catenin (ABC). Data are representative of at least three independent replicates.