Fig. 3. Gene knockdown of CK1/ reduces the phosphorylation of Dvl2 induced by Wnt-5a. (A) SN4741 cells were transfected with control siRNA and three independent siRNAs against CK1 isoform. The efficiency of gene knockdown induced by individual siRNAs against CK1 was quantified in western blots. A non-specific protein, resulting in a band recognised by anti-CK1 antibody served as a loading control. Quantification (normalised to loading control) is shown below. (B,C) SN4741 cells were transfected with the indicated combinations of siRNAs, and treated with control, 50 and 100 ng/ml of Wnt-5a. The phosphorylation of Dvl isoforms was detected as phosphorylation-dependent mobility shift of total Dvl2. Data are representative of three (B) or two (C) independent experiments. The position of dephosphorylated () and phosphorylated Dvl () is indicated. The levels of CK1 and -actin were also determined to confirm the efficient gene knockout and the equal protein loading.