Fig. 5. Pathogen attack promotes cytosolic Fe depletion, leading to defense gene expression. (A) RT-PCR of mRNAs from epidermal (e) and mesophyll (m) tissues of wheat leaves before (0 hpi) and after (24 hpi) inoculation with Bgt. TmGPD1 was used to check equal mRNA usage. (B) Northern blot analysis of TmNAS1 and TmPR1b expression in wheat leaves 0-72 hpi with Bgt. (C) Northern blot analysis of total RNA from wheat leaves treated with either DFO alone or DFO followed by H₂O₂ for either 6 or 18 hours.