Fig. 6. H₂O₂ promotes cytosolic Fe depletion and Fe efflux. (A,B) Confocal imaging of Fe depletion in wheat suspension cells, based on calcein-AM fluorescence. Images (lower panels) depict the fluorescence at representative time points. Scale bar, 20 µm. Time course of calcein-AM uptake (A); H₂O₂-mediated cytosolic Fe depletion (B). (C,D) H₂O₂-induced Fe efflux in wheat suspension cells. Bar values represent mean ± s.d. (n=3). Cells were treated with glucose/glucose oxidase (G/GO) (5 mM/5 U ml^–1) or H₂O₂ (10 mM), with or without catalase (Cat) (10 U ml^–1) or vanadate (NaVO3) (100 µM), respectively (C); cells treated with 10 mM H₂O₂ and 20 or 200 µM cycloheximide (CHX) (D).