Fig. 1. Distribution of PRMT1 and hCAF1 in MCF-7 cells. Confocal fluorescence microscopy experiments showing the subcellular distribution of endogenous PRMT1 and hCAF1. (A) PRMT1 was visualized using a monoclonal anti-PRMT1 antibody in MCF-7 cells (a) control or transfected with (b) scramble siRNA duplexes or (c) specific siRNA PRMT1 duplexes. The corresponding phase-contrast micrographs are shown in panels a'-c'. (B) Suppression of endogenous PRMT1 was checked by western blotting with polyclonal anti-PRMT1. A loading control was performed with an anti-GAPDH antibody. (C) Immunofluorescence analysis using a rabbit polyclonal anti-hCAF1 antibody in MCF-7 cells (a) control or transfected with (b) scramble siRNA duplexes or (c) specific siRNA hCAF1 duplexes. The corresponding phase-contrast micrographs are shown in panels a'-c'. (D) Suppression of endogenous hCAF1 was checked by western blotting. A loading control was performed with an anti-GAPDH antibody.