Fig. 5. IGF-1 induces an increase in the DNA binding activity of the NFAT transcription factors and a nuclear translocation of NFATc2. (A) The NFAT DNA binding activity of the cultures treated by IGF-1 was investigated by EMSA using the consensus NFAT sequence from the IL-13 promoter. A competition with the unlabelled competitor oligo, a mutated probe and supershift using panNFAT antibody or antibodies directed against NFATc1, NFATc2, NFATc3 or NFATc4 were performed. (B) Immunostaining using sera directed against NFATc1, NFATc2, NFATc3 or NFATc4 isoforms was performed after IGF-1 treatment. Nuclei were counterstained with Hoechst. ^*P<0.05.