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Figure 5


Fig. 5. The 19S RP base in the ATP-bound conformation mediates proteasome binding to the ER. Wild-type 19S RP (1 pmol) or 19S RP with the indicated mutations was incubated with 10 eq dog PK-RM and analyzed by sucrose gradient centrifugation, SDS-PAGE and immunoblotting for the FLAG-tagged Rpn11p subunit. Positions of membrane-bound and unbound 19S RP are indicated.