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Figure 2


Fig. 2. Static stretch activates myogenic differentiation markers. C2C12 myoblasts were stretched for the indicated time periods as described in Fig. 1. Whole cell extracts were prepared and subjected to western blot analysis for phosphorylated MEF2C and total MEF2C (46 kDa), myogenin (37 kDa) and p21 (21 kDa). Total MEF2C also serves as a control for the sample loading quality of myogenin and p21 blotting.