Fig. 5. clb2^NLS cells display a premature switch of growth polarization. (A) Cell cycle regulation of Bem1-GFP localization. (B) The percentage of cells with Bem1-GFP polarized at the bud tip was monitored in the indicated strains in log-phase cultures at 25°C. Medium and large buds were 2,5-3,5 µm and 3,5-5 µm in length respectively. Large budded cells with Bem1-GFP localized at the mother-bud neck were not scored. Bars indicate the standard deviation of at least three independent experiments with more than 500 cells counted for each strain. (C) Cells from the indicated strains were arrested in G1 with 5 µg/ml -factor, washed and synchronously released in fresh YPD medium containing 15 µg/ml nocodazole. The cells were fixed after 75 minutes at 30°C and processed for actin staining with Rhodamine-phalloidin. The percentage of cells displaying a polarized distribution of cortical actin patches was quantified for each strain by counting at least 150 cells. Bars, 2 µm.