Fig. 6. LINT-25 protein levels change during the cell cycle of proliferating cells. (A) BJ1 cells at different phases of the cell cycle were prepared by centrifugal elutriation and cell fractions were analyzed by DNA flow cytometry. Cell lysates were processed for immunoblotting using antibodies against LINT-25 and LAP2. FACS profiles from representative fractions and from an asynchronous culture (log) are shown. (B) WI-38 human lung fibroblasts were synchronized by an overnight aphidicolin block and released from the block for indicated time periods. Samples were processed for immunoblotting after the indicated time points and analyzed using antibodies against LINT-25, LAP2 and actin. The first lane (–) is a sample from untreated control cells; numbers on the right are molecular masses of marker proteins in kDa; mLINT-25, monomeric LINT-25; dLINT-25, dimeric LINT-25.