Fig. 7. LINT-25 acts upstream of LAP2. (A) Primary skin fibroblasts from LAP2 knock-out mice were passaged in culture until senescence (S) and processed for immunofluorescence microscopy together with proliferating control cells (P) using antibodies against LINT-25, LAP2 or Ki67. (B) GFP-tagged LINT-25 (upper panel) or eGFP alone (lower panel) were transiently expressed in BJ1 cells and samples were processed for immunofluorescence microscopy 15 hours after transfection using antibodies against Ki67, LAP2 and Hoechst dye (blue). Confocal images are shown. Bars, 10 µm.