Fig. 5. Expression of IL-1 proteins in HEK 293 cells. (A) HEK 293 cells were transfected with empty vector (C), and constructs encoding Flag-labeled pro-IL-1 (31), Flag-labeled 27-kDa IL-1 (27) and Flag-labeled mature, 17-kDa IL-1 (17). Cells were immunostained for IL-1 (green) and cathepsin D (CD, red). Bar, 10 µm. (B) IL-1 immunoblot of HEK 293 cell lysates transfected with empty vector (C), and constructs encoding Flag-labeled pro-IL-1 (31), Flag-labeled 27-kDa IL-1 (27) and - Flag-labeled mature, 17-kDa IL-1 (17). Images and blots are representative of three independent experiments.