Fig. 6. Blebbing macrophages retain their IL-1
. (A) Wide-field fluorescence images showing IL-1
in LPS-treated (1 µg/ml, 2 hours) murine macrophages stimulated with ATP (5 mM) for the indicated times in minutes (min). Bar, 10 µm. (B) High-resolution, deconvolved images showing IL-1
in LPS-treated (1 µg/ml, 2 hours) murine macrophages stimulated with ATP (5 mM) for the indicated times. Bar, 5 µm. These images are representative of three independent experiments.