Fig. 5. The distribution of focal adhesion proteins in Ehd1^–/– MEF cells is altered at steady-state. MEF cells were immunostained with antibodies to FAK (A,B), paxillin (C,D) or phospho-paxillin (E,F) and analyzed by confocal microscopy. Lysates containing an equal amount of protein from Ehd1^+/+ and Ehd1^–/– MEF cells were immunoblotted with antibodies against actin, FAK, phosphorylated FAK, paxillin or phosphorylated paxillin (G). Bar, 10 µm.