Fig. 3. Association of Ptc with the Hh complex is independent of Sxl. (A-F) Male salivary gland cells with the Smo^D16 deletion allele (and PKA^H2). Ci and Ptc colocalize at the plasma membrane and vesicular network (C and F colocalized pixels only). (G) Adult male and female extracts treated with anti-Ci and Cos2 show that Cos2 immunoprecipitates 16% of the Ptc in males, 22% in females; Ci immunoprecipitates 1% of the Ptc in males and 16% in females.