Fig. 5. Colocalization of Ptc and Smo, with Ci and Sxl. Wild-type female and male salivary gland cells stained for Ci and Ptc or Ci and Smo (and Sxl in females) in the presence and absence of Hh. Female glands in the absence of Hh stained for Sxl, Smo and Ci (A-C) or Sxl, Ptc and Ci (G-I). Male glands in the absence of Hh signal stained for Smo and Ci (D,E) or Ptc and Ci (J,K). Female glands expressing Hh stained for Sxl, Smo and Ci (M-O) or Sxl, Ptc and Ci (S-U). Male glands expressing Hh stained for Smo and Ci (P,Q) or Ptc and Ci (V,W). `Col.' panels (A'-C',F,G'-I',L,M'-O',R,S'-U',X) show only the pixels that are common between two proteins. Note the extensive colocalization close to the plasma membrane in most panels.