Fig. 7. Colocalization percentages of Ci and Sxl with Ptc and Smo in both sexes. (A) Colocalization of full-length Ci with the two Hh membrane components, Ptc and Smo (1), as well as Sxl (2), in wild-type salivary glands in the absence (–Hh) and presence of Hh (+Hh) at the plasma membrane (PM) and within the cell. Colocalization of the proteins at the plasma membrane (B) and within the cell (C), in salivary glands of the wild type or animals expressing Shi, Rab5 or Rab7 mutant proteins. Males and females show differences in amounts. Plasma membrane optical sections include region near the membrane. Sections taken within the cell include a cross section of the nucleus. Percentages reflect the average of at least two, usually three separate optical sections.