Fig. 8. Ptc and Sxl are endocytosed with Hh signaling components. Ci, Cos2, Smo or Sxl IP of embryo (0- to 12-hour) extracts expressing the dominant negative Shi (Shi^K44A), Rab5 (Rab5^SN) or gain-of-function Rab7 (Rab7^QL) variant probed for Ptc and Sxl. Percentage of Sxl (A) and Ptc (B) immunoprecipitated for each variant compared with wild-type (wt) data of Fig. 1. The average and standard error are the mean of two or more data sets. Error bars represent ±1 s.e. White bars, wild type; light gray bars, Shi; white/black dot bars, Rab5; dark gray bars, Rab7.