Fig. 5. Effect of rotenone treatment on mitochondrial , cytosolic ROS and matrix redox potential. (A) Determination of in MRC5 cells treated with increasing concentrations of rotenone, measured with the JC1 probe and expressed as the ratio of red to green fluorescence. (B) Determination of the cytosolic ROS concentration by fluorescence intensity of the H₂-DCFDA probe, expressed as the percentage of control value. Results are given as the mean ± s.d., n=3. (C) Measurement of the redox potential of the mitochondrial matrix in MRC5 cells treated with increasing rotenone concentrations, measured with redox-GFP and expressed as the percentage of the fluorescence intensity (emission ratio of 511 nm, excitation set at 400 nm and 480 nm) measured in control cells (not shown). ^*P<0.05, statistically significant change compared with the control.