Fig. 7. Laminin stimulates increased binding of Rho to the Rho-binding domain of Rhotekin in C2 myotubes. Laminin-induced Rho activation in nontransfected C2 myotubes was measured by the increase of endogenous Rho bound to GST-TRBD. Cultures were treated with laminin (10 nM) for 15 minutes, and lysates were incubated with GST-TRBD and western blotted with antibody to Rho. Laminin caused increased association of Rho with GST-TRBD in myotubes that is similar to that activation of Rho seen with agrin.