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Figure 1


Fig. 1. Decay of the proliferation capacity of ES cell-derived- and neonatal cardiac cells after cell isolation from embryoid bodies and neonatal mouse hearts. (A,B) Decay of the numbers of BrdU-positive cell nuclei of cardiac cells. (C,D) Decay in Ki-67 immunoreactivity in cardiac cells. (E,F) Nuclear/cytoplasmic (n/c) cyclin D1 distribution in cardiac cells. (G,H) Decay in retinoblastoma (Rb) phosphorylation in cardiac cells during different times of cell culture. (I,J) Increase in p27kip1 expression in the cell nuclei of cardiac cells during different times of cell culture. *P<0.05, significantly different to day 1 of cell culture.