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Figure 3


Fig. 3. Effects of opioid receptor stimulation on DEPV-induced cardiomyocyte apoptosis. (A) Representative electrophoretic analysis of nucleosomal DNA fragmentation from six experiments, showing the effect of morphine or DADLE on DEPV-induced cardiomyocyte apoptosis in the presence or absence of the nonselective-, {kappa}-, or {delta}-opioid receptor antagonist. (B) Representative Hoechst 33258 staining from six experiments, showing morphine-induced protection against DEPV-induced cardiomyocyte apoptosis. a, control; b, DEPV; c, DEPV + MOR; d, DEPV + MOR + NAL. Bar, 20 µm. (C) The effect of morphine or DADLE on DEPV-induced cardiomyocyte apoptosis in presence or absence of the nonselective-, {kappa}-, or {delta}-opioid receptor antagonist. Bar chart shows the percentage of apoptotic cells, as assessed by TUNEL staining, in each group (n=6 in each group). Values are the mean ± s.d. *P<0.05 among the indicated groups. DEPV, serum and glucose deprivation; DADLE, [D-Ala2, D-Leu5]-enkephalin acetate salt; NAT, natrindole; MOR, morphine; NAL, naloxone; GNTI, guanidinyl-naltrindole.