Fig. 9. Survivin overexpression protected DEPV-induced apoptosis but not ROS production and mitochondrial membrane depolarization. (A) Western blots analysis showing high levels of the GFP-survivin fusion protein in the cardiomyocytes transfected with GFP-survivin vectors but not in the cells transfected with GFP control vector. (B,C) Representative micrographs (B) and quantification of apoptotic cardiomyocytes expressed as the ratio of the TUNEL positive cells to GFP positive cells (C). (D,E) Survivin overexpression had no effect on ROS production (D) and mitochondrial membrane potential depolarization (E). (F) Glybenclamide was not effective in preventing DADLE-induced increase in survivin expression. Values are the mean ± s.d. ^*P<0.05. n=6 in each group. DEPV, serum and glucose deprivation; DADLE, [D-Ala2, D-Leu5]-enkephalin acetate salt; GFP, green fluoresce protein; TUNEL, TdT-mediated dUTP nick end labeling; ROS, reactive oxygen species.