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Figure 2


Fig. 2. Overexpression of SMA2 partially suppresses the sporulation defect of an erv14{Delta} mutant. (A) Sma2p-GFP localizes to the prospore membrane. Sma2p-GFP expressed from pRS424-SPO20pr-SMA2-GFP was observed in a sporulating wild type cell (AN120). DNA was stained with DAPI (DAPI). Bar, 1 µm. (B) Overexpression of SMA2 restores sporulation to the erv14{Delta} mutant. erv14{Delta}/erv14{Delta} (H126) cells harboring pRS314-ERV14HA, pRS424-SPO20pr-SMA2 or empty vector, were sporulated and 3x105 cells were spotted onto a YPD plate, exposed to ether vapor and then incubated at 30°C.