Fig. 6. Expression of the metalloproteinase Mmp2 in the whole wing disc reproduces the phenotype resulting from a lack of integrin function. Staining of late third-instar wing discs with Rhodamine-phalloidin (A,B,A',B') show that ectopic expression of Mmp2 in the whole wing pouch (B,B') leads to the formation of extra folds. This can be clearly seen in xz confocal sections (A',B'). The white line in A and B indicates the position of the z section. (C) Scanning microscopy show that in some cases these extra folds seem to extend and form a pouch. (D,E) Confocal sections through the basal side of the wing pouch area of discs stained with an anti-PS antibody (green) show that the basal distribution of the endogenous PS integrins found in wild-type discs (D) is not affected upon Mmp2 overexpression (E). (F) Histological cross-sections show that expression of Mmp2 leads to a reduction in cell height (arrow indicates the height of the wing epithelium), resulting in wing blistering in the adult (G).