Fig. 7. Expression of di inhibits endogenous integrin localisation while recruiting talin to the basal membrane. (A,B) Late third-instar wing discs expressing di (green in inset in A) in a narrow band of cells along the dorsoventral axis of the wing disc using the patched-GAL4 line (ptcG4). (A) Confocal sections through the basal side of wing discs stained with an anti-PS antibody show that the expression of di affects the basal localisation of the endogenous integrins. (B) However, z sections show that the PS protein is still present on the lateral and apical surfaces. The white line in A indicates the position of the z section. (C-E) Confocal sections of late third instar wing imaginal discs expressing di in the posterior (p) compartment, stained for the PS subunit (green) and talin (red). In the anterior (a) wild-type half, talin colocalises with the PS subunit in basal focal adhesion-like structures (C). In the posterior compartment, di inhibits PS clustering (D), but not talin recruitment (E). In fact, di recruits talin more efficiently than the endogenous integrins (E, compare levels of talin in p with those in a). Bars, 30 µm (A), 20 µm (C-D).