Fig. 9. Raf^DN suppressed the effects of di on receptor localisation and matrix integrity. (A-D) Confocal sections of wing imaginal discs expressing di (A,C) or di+Raf^DN (B,D) using the patch-GAL4 line (ptcG4) (inset in A). (A) Staining for PS (green) shows that expression of di inhibits integrin recruitment to focal adhesions. (B) Coexpression of Raf^DN in these cells substantially rescues endogenous integrin distribution. (C,D) Coexpression of Raf^DN also suppresses the ability of di to disorganise the basal matrix (green), compare the appearance of the matrix within the patch domain in C with that in D. Bar, 20 µm. (E) In wild-type larval third-instar discs MAPK activation is detected specifically in all veins and in the wing margin. (F) In wing imaginal discs expressing di using the engrailed-GAL4 line (enG4) MAPK activity is now detected in the whole posterior compartment.