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Figure 1


Fig. 1. The ER concentrates in the oocyte during oogenesis and is derived from fusome membranes representing a specialized ER. (A-D) The ER accumulates in the oocyte during oogenesis, as shown using an antibody against (A) the ER-retention signal HDEL and three different GFP-tagged ER proteins: (B) Rtnl1-GFP, (C) protein disulfide isomerase (PDI) and (D) translocon channel subunit Sec61{alpha} (Sec61-{alpha}GFP). Arrows indicate HDEL labeling of the fusome in the germarium. Note that only Rtnl1-GFP is specifically enriched in the germline and mostly absent from the somatic follicular cell layer. (E) Concentration of Rtnl1-GFP in the fusome during different stages of cyst maturation in the germarium (for a scheme of stages within a germarium see Fig. 2A). Bars, 50 µm (A), 10 µm (B-E).