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Figure 2


Fig. 2. P-T295 of Xenopus Aur-A is not detected until GVBD. (A) Protein extracts from oocytes collected after incubation in progesterone (hr in pg) for the indicated times (in hours) were analyzed by SDS-PAGE and immunoblotting with antibodies against total Aur-A (top panel) and P-T295 Aur-A (P-Aur-A, bottom panel). (B) Protein extracts from oocytes collected at 100% GVBD were immunodepleted with either anti-Aur-A antibody or non-specific IgG. Samples were analyzed by SDS-PAGE and immunoblotting with antibody for either P-T295 Aur-A (P-Aur-A, top panel) or Aur-A (bottom panel). Arrowheads indicate migration of mobility shifted Aur-A;? indicates the band of a protein detected non-specifically by the antibody.