Fig. 5. Cellular levels of repair replication factors are markedly reduced in quiescent cells. (A) MSU-2 or XP2BI cells (AS or G0) were lysed and cellular levels of the indicated proteins were analyzed by immunoblotting with specific antibodies. (B) Growth-arrested MSU-2 cells were released as described in the legend of Fig. 1C. Cell lysates were prepared at the indicated time points and used for immunoblotting with anti-DNA Pol
(125-kDa CS) or anti-DNA Pol
(261-kDa CS) antibodies. The quantitative data are shown in the bottom panel and represent the relative protein levels (%) to the AS sample.