Fig. 3. Correlative light and electron microscopy of filopodia-associated veil protrusion and retraction. (A) Phase-contrast panels show a low magnification image of the growth cone with a white box denoting the region of interest, followed by four consecutive images from the last 9 seconds of the time-lapse sequence before the cell was extracted, a live-live phase-contrast merge of the first and last images, the same region after extraction `lysed', and a phase-contrast merge of the lysed and the last live image. In all merges, red regions indicate protrusion, cyan regions indicate retraction. The veil in the left panel was protruding at 4.2 µm/minute, the veil in the right panel was retracting at 1.7 µm/minute. (B) Electron micrographs of region of interest from the time lapses in A. (C) Higher magnification of the boxed regions in B.