JCS -- Rey et al. 120 (6): 1126 Figure IG1

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Fig. 1. Confocal microscopy analysis of SDF-1 ${alpha}$ -induced endocytosis of MIIA and CXCR4. (A) Confocal analysis of MIIA- and CXCR4-staining in PBLs untreated or treated with 300 nM SDF-1 ${alpha}$ for the indicated time periods. Quantification of the fluorescence signal of CXCR4 and MIIA in the cytosolic compartment versus the plasma membrane was performed on the complete 3D confocal stack for n=10 cells from two independent experiments, and represented as the mean percentage of internalization ± s.d.; ^*P<0.05. (B) Confocal analysis of MIIA, CXCR4 and CD45 in J77 T cells untreated or treated with 300 nM SDF-1 ${alpha}$ for the indicated periods of time. CD45 is depicted as a control of plasma membrane molecule that is not internalized in response to SDF-1 ${alpha}$ . Bars, 5 µm.