Fig. 1. ADMA induces formation of stress fibres and focal adhesions in PAECs and inhibits cell motility. (A-D) Control untreated cells; (E-H) cells treated with 100 µM SDMA; (I-L) cells treated with 100 µM ADMA for 24 hours. (A,E,I) Phase contrast microscopy images showing sparsely growing PAECs. (B,C,F,G,J,K) Fluorescent images showing staining for F-actin and a focal adhesions component vinculin. (D,H,L) Representative images of cell trajectories. Bar, 100 µm (A,E,I), 10 µm (B,C,F,G,J,K) and 130 µm (D,H,L). (M) Changes in the mean speed of cell movement in untreated cells, cells treated with SDMA (100 µM), ADMA (100 µM) or the cells grown in arginine-free medium with or without ADMA. (N) Total cell translocation defined as a distance between starting point and an end point of each trajectory. ^*P<0.05; ^**P<0.01 (comparison with untreated controls).