Fig. 10. Pulmonary microvascular endothelial cells from DDAHI heterozygous knockout mice (HT) show phenotypical and functional differences from cells taken from their wild-type (WT) littermates. (A,B) Phase contrast microscopy images; (C,D) fluorescent confocal images of F-actin in WT and HT cells, as indicated. (E,F) Cell trajectories, 24 hour recording. Bar, 50 µm (A,B), 20 µm (C,D) and 120 µm (E,F). (G) Changes in nitrite levels in culture medium of cells from wild-type (WT) mice, heterozygous DDAHI knockout (HT) mice, and cells from WT mice treated with ADMA (100 µM); (H,I) changes in speed and translocation of these cells. (J) Graphs and western blots showing activities of RhoA, Rac1 and Cdc42 in cultured PMVECs taken from WT and HT mice.^**P<0.01; ^*P<0.05 (comparison with WT controls).