Fig. 5. The effects SNAP and L-NAME on the actin cytoskeleton, focal adhesion formation and cell movement in PAECs. (A-C) Cells incubated with 10 µM SNAP for 4 hours; (D-F) cells incubated with ADMA (100 µM) and SNAP (10 µM) for 24 hours; (G-I) cells incubated with L-NAME for 24 hours. The columns from left to right show F-actin distribution in cells, vinculin distribution and cell trajectories, as indicated. Bars, 15 µm (A,B,D,E,G,H) and 170 µm (C,F,I).