Fig. 6. The effects of SNAP and L-NAME on cell speed (A), translocation (B) and nitrite levels (C). SNAP (10 µM) and L-NAME (1 mM) were added to untreated cells or cells treated with ADMA and incubated for 24 hours. Nitrite levels in culture medium were measured using a standard Griess assay. In (D) two sets of corresponding images show localisation of endogenous DDAHI in normal PAECs. The cells were stained for F-actin (left) and DDAHI (right). White arrows point to colocalisation of F-actin and DDAHI in membrane ruffles and lamellipodium. Bar, 8 µm. (E) shows expression of DDAHI in untreated PAECs (western blot on the left). Differences in expression levels between DDAHI and DDAHII in PAECs are also shown in a graph and a corresponding western blot on the right.