Fig. 7. The effect of L-NAME and SNAP on the activity of RhoA. (A,B) Graphs show RhoA activity in cells treated with (A) L-NAME (0.5 mM and 1 mM) for 24 hours, or (B) treated with L-NAME (1 mM) for 4 and 24 hours. (C) Graph shows time-dependent changes in RhoA activity in cells treated with SNAP (10 µM). (D) Graph shows changes in Ser188 phosphorylation of RhoA in cells treated with ADMA (100 µM), SDMA (100 µM), SNAP (10 µM) or SNAP and ADMA. ADMA and SDMA were added to the cells for 24 hours whereas SNAP was added for 4 hours. Representative examples of western blots are shown below the graphs. Values in A and C are means of three experiments whereas values in B are means of two experiments. ^*P<0.05, ^**P<0.01 (comparison with untreated controls).