Fig. 8. The effects of overexpression of non-phosphorylatable mutant of RhoA, Ala188RhoA in PAECs. The cells were stained for F-actin (A,D), vinculin (B,E) and haemagglutinin (C,F) 24 hours after transfection. Bar, 10 µm. (G) Western blot of control cells and cells overexpressing Ala188RhoA-HA. The blot was probed for RhoA and haemagglutinin (HA), as indicated. (H,I) Cell speed and translocation in control, untreated cells, cells treated with 100 µM ADMA, cells transfected with an empty vector (transfection control) and cells transfected with Ala188RhoA. ^**P<0.01 (comparison with untreated controls).