Fig. 9. The effects of the PKG activator, Br-cGMP, and PKG inhibitor, Rp-cGMPS, on untreated and ADMA-treated PAECs. (A-H) The effects of Br-cGMP and Rp-cGMPS on F-actin and vinculin distribution, in untreated cells and ADMA-treated cells, as indicated. Bar, 10 µm. (I,J) The effects of the drugs on cell speed and translocation, respectively. (K) Changes in RhoA activity in cells treated with ADMA (100 µM), ADMA (100 µM) and Br-cGMP (500 µM), Br-cGMP (500 µM) alone or Rp-cGMPS (100 nM). A representative example of a western blot is shown beside the graph. In A-H and K, ADMA was added to the cells for 20 hours while Br-cGMP and the Rp-cGMPS were added for 2 hours. In motility assays (I,J) all drugs were added to the cells at the same time and incubated for 24 hours. Values in I,J are means of three experiments whereas values in K are means of two experiments. ^**P<0.01 (comparison with untreated controls). ^&P<0.05 and ^&&P<0.01 (comparison with ADMA-treated cells).