JCS -- Rasmussen et al. 120 (6): 953 Figure IG5

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Fig. 5. Overview of AIS localization and current levels for the various KCNQ constructs. (A) Summary of observed KCNQ AIS localization in neurons transfected with the listed combinations of constructs. AIS/distal axon (AIS/DA) and AIS/dendrite (AIS/den) ratios were determined for each combination of constructs for 29-30 cells in at least three different cultures. Q2+Q3 ${Delta}$ was statistically different from wild-type Q2+Q3 for both KCNQ2 and KCNQ3 immunofluorescence quantifications, whereas Q2 ${Delta}$ +Q3 was not. In addition, Q2+Q3 ${Delta}$ was statistically different from Q2 ${Delta}$ +Q3 ${Delta}$ in the KCNQ2 quantification, but not for the KCNQ3 quantification. One-way ANOVA and Bonferroni's post-test were used in the comparison of Q2+Q3 vs Q2 ${Delta}$ +Q3, Q2+Q3 vs Q2+Q3 ${Delta}$ and Q2 ${Delta}$ +Q3 vs Q2+Q3 ${Delta}$ (^*P<0.05, ^**P<0.001). (B) Summary of current density levels observed in neurons transfected with the listed constructs. All neurons were co-transfected with GFP. Recordings were carried out on cultures from at least two different days. Q2+Q3 and ${Delta}$ Q2+ ${Delta}$ Q3 were statistically different from controls. One-way ANOVA and Dunnet's multiple comparison post-test were used to compare the various constructs with the control (^*P<0.05). (A+B) Data are displayed as mean±standard error of mean. Abbreviations: control, GFP; Q2, hKCNQ2-cmyc; Q3, hKCNQ3-FLAG; Q2 ${Delta}$ , hKCNQ2-delAnkG; Q3 ${Delta}$ , hKCNQ3-delAnkG; Q2A, Q2-AAA; and Q3-A, Q3-AAA.