Fig. 6. BMP-9 attenuates migration and inhibits proliferation of ECs. (A) BAECs were allowed to grow to confluence and serum-starved overnight. Monolayers were wounded and stimulated with either 30 ng/ml bFGF or 10 ng/ml BMP-9. Wound closure was measured after 24 hours using the Olympus Analysis software. (B) Scratched BAEC monolayers were incubated with 10 ng/ml BMP-9 and different concentrations of bFGF as indicated in the figure and cell migration was measured after 24 hours. (C,D) 3000 BAECs were seeded in 96-well plates and stimulated with different concentrations of BMP-9, 30 ng/ml bFGF or combinations of bFGF and BMP-9. Cell proliferation was determined after 2 and 3 days by adding the MTS reagent and measuring the absorbance at 490 nm. B9, BMP-9. ^##P<0.001 compared with bFGF treatment; ^#P<0.01 compared with control; ^**P< 0.005 and ^*P<0.05 compared with the respective bFGF treatment without BMP-9.