Fig. 1. Recruitment of EGFP-Rab3A and EGFP-Rab27A to newly synthesised granules in PC12 cells. The cells were examined 18 hours after transfection. (A-C) Cells after fixation co-immunostained with anti-Rab3A and anti-secretogranin II (anti-SGII). (D-F) Cells co-transfected with plasmids encoding ECFP-Rab27A and EGFP-Rab3A. (G-I) Cells transfected with a plasmid encoding ECFP-Rab27A, which after fixation, were immunostained with anti-SGII. (J-L) Cells cotransfected with plasmids encoding ECFP-Rab27A and prepro-atrial natriuretic factor-EGFP (ppANF-EGFP). Each overlay image on the right of the figure is a composite of green from the left image and red from the centre image. Areas of overlap appear in yellow. Magnified views of areas of colocalisation are shown at the bottom right. Bars, 4 µm.