JCS -- Peter et al. 120 (6): 996 Figure IG4

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Fig. 4. DGC proteins are increased upon SSPN overexpression. Immunoblot analysis of total proteins extracted from skeletal muscle of non-Tg (lane 1), non-phenotypic SSPN-Tg line 29.1 (lane 2), and phenotypic SSPN-Tg line 37.5 (lane 3) mice. Skeletal muscle was solubilized using 3% SDS buffer and protein samples (60 µg) were separated by SDS-PAGE and transferred to nitrocellulose membranes. Equal loading of protein samples was confirmed by Coomassie Blue (CB) stain. Immunoblots were probed with antibodies to laminin (Lam), dystrophin (Dys), ${alpha}$ - and $beta$ -DG, ${alpha}$ -, $beta$ -, and ${gamma}$ -SG, exogenous SSPN (hSSPN), endogenous SSPN (mSSPN) and caveolin-3 (cav-3), as indicated.