Fig. 4. S10A synapsin redistributes Glut4 in adipocytes. L1 adipocytes prepared as described in Fig. 3 were treated with or without insulin (100 nM, 30 minutes), then fixed and analyzed. zstack confocal images of GFP fluorescence were collected from 60 infected adipocytes of each type and maximum projections were analyzed. (A) Representative images. (B) Percentage of cells in the maximum projections with Glut4 at or near the plasma membrane (rim + diffuse fluorescence) in basal (white) or insulin-stimulated (black) cells. (C) Average object sizes (contiguous area with green intensity greater than the threshold in maximum projections; mean ± s.e.m., standardized to Glut4 basal). (D) Histograms of intensity distributions (shown on z-axis) of GFP fluorescence in the representative images shown in A (G4, G4/S, G4/S10A, left to right).