Fig. 1. SENP5 regulates mitochondrial fission. (A) COS-7 cells transfected as indicated were fixed and examined for mitochondrial morphology by examining the OCT:CFP signal. Values represent the mean ± s.d. of three coverslips, examining 50-100 cells from each. Fragmented, mitochondria with a stokes radius of 1. (B) Representative images of the OCT-CFP matrix marker shown as an example of the rescue phenotype. (C) Lysates of COS-7 cells (transfected as indicated) examined by SDS-PAGE, and then probed with anti-His6, anti-FP, anti-DRP1, anti-Mfn2 and anti-Tom20 antibodies. (D) Quantification of DRP1 protein levels obtained from the blots in C was expressed as a percentage of the intensity found in cells transfected with SUMO1:His alone. The values were normalized to the levels of Tom20 in each lane to ensure equal loading (n=3). UTF, untreated cell fraction.