Fig. 4. Dnr1 depletion sensitizes cells to induction of apoptosis. Control S2 cells, GFP dsRNA-treated S2 cells or Dnr1 dsRNA-treated S2 cells were incubated with 0.01, 0.1 or 1 µM actinomycin D (A-C, respectively) and the apoptotic index of the different cell populations were determined as indicated. Results are the mean of three independent experiments and error bars indicate standard errors. ^**P<0.01, values that differ significantly from Dnr1 dsRNA-treated cells; ^*P<0.05, values that differ significantly from Dnr1 dsRNA-treated cells. Dnr1 RNAi results in an elevated apoptotic index of actinomycin-D-treated S2 cells compared with control S2 cells or GFP dsRNA-treated S2 cells.