Fig. 3. FISH localization of repetitive sequences in leaf and protoplasts. DAPI staining (left column); FISH signal (middle column); merge (right column). (A,B) The repeats of the pericentric BAC F28D6 localize at chromocenters in leaves (A) but are dispersed in protoplast nuclei (B). (C,D) The Athila transposon localizes at chromocenters in leaves (C) but is dispersed in protoplasts (D). (E) Both 5S (red) and 45S (green) rDNA repeats localize at chromocenters in leaves. (F) In protoplasts, 5S rDNA shows decondensed signals, whereas 45S rDNA signals remain condensed at the chromocenters. (G,H) The 180 bp centromere repeat localizes in chromocenters of leaf nuclei (G), but is dispersed in protoplast nuclei (H). Bar, 5 µm.