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Figure 5


Fig. 5. DNA methylation pattern in nuclei and in repeat regions. (A) DAPI staining (upper panels) and immunolocalization of 5-methylcytosine (lower panels) in leaf (left), protoplast (middle) and cultured cell (right). Bar, 5 µm. (B) Southern blot analysis of leaf, protoplast (pp) and cultured (cc) cells. DNA was digested with MspI (M) and HpaII (H). The blots were hybridized with DNA probes from the 180 bp repeat, 45S rDNA and Athila transposon.